One and Only Live Nitrifying Bacteria for Ponds• Immediately works to condition new pond filters • Instantly creates a biofilter • 100% living, nitrifying bacteria • Removes toxic ammonia and nitrite, naturally Good for 6 months at room temperature, 1 year at 50°F. Best to keep refrigerated for longest shelf life. One and Only Live Nitrifying Bacteria works quickly to condition new pond by instantly seeding the biofilter with billions of live nitrifying bacteria. Use One and Only to control ammonia and nitrite levels. One and Only Live Nitrifying Bacteria has no sulfur or other offensive odors. Directions for Use: SHAKE WELL BEFORE USING BEST TO USE THE ENTIRE CONTENTS AT ONE TIME Minimum dose 10 ml (2 capfuls) per 10 gallons of pond water. One and Only Nitrifying bacteria cannot be overdosed. 32 OZ Treats 480GAL or 1817L